(DOWNLOAD) "Purification and Characterization of Lactate Dehydrogenase from the Heart Ventricles of River Buffalo (Bubalus Bubalis) (Report)" by Pakistan Journal of Zoology " eBook PDF Kindle ePub Free
eBook details
- Title: Purification and Characterization of Lactate Dehydrogenase from the Heart Ventricles of River Buffalo (Bubalus Bubalis) (Report)
- Author : Pakistan Journal of Zoology
- Release Date : January 30, 2011
- Genre: Life Sciences,Books,Science & Nature,
- Pages : * pages
- Size : 179 KB
Description
Byline: Muhammad Shahid Nadeem, Atif Nissar, Saher Shahid, Ayesha Imtiaz, Muhammad Mahfooz Muhammad Tahir Asghar and Abdul Rauf Shakoori Abstract The present study describes purification and characterization of lactate dehydrogenase (LDH) from the heart ventricles of river buffalo (Bubalus bubalis). Heart specific isozyme of LDH has been purified to apparent homogeneity on SDS-PAGE using ion-exchange column chromatography, selective precipitation in the presence of ammonium sulfate and hydrophobic-interaction chromatography. The enzyme was purified up to 48 fold with 16% recovery. The maximum activity of purified enzyme was observed at pH 7.0 and it has shown reasonable stability at a broad range of temperature with maximum activity at 30degC. The Km value with pyruvate is 41 uM, it has only 18% activity with lactate as compared to its activity with pyruvate at pH 7.0. The molecular weight of a subunit of enzyme is 36416.5+-2 Da as determined by MALDI-TOF analysis.
 "Purification and Characterization of Lactate Dehydrogenase from the Heart Ventricles of River Buffalo (Bubalus Bubalis) (Report)" by Pakistan Journal of Zoology " eBook PDF Kindle ePub Free){kind=link}